This thesis emphasizes the definition of new modalities and frameworks for cooperation of entities in tourism, in order for those entities to survive in the tourism market, strengthen their intermediating role and achieve their business goals. The tourism sector has experienced growth in the recent years, as seen in all of its business segments. The developments in technology make the supply and demand of the market change accordingly, as it is necessary to keep up with innovations. These constant changes in the market also cause changes in the way the holders of tourism activities do business, as seen through the business integrations, the use of new technological platforms and continuous investments in training programs and employee education. Therefore, this thesis puts a particular emphasis on the challenges contained in the factors and the intensity of their impact on the development of intermediaries, and the new modalities of their integration and business cooperation. By doing so, tourist intermediaries show willingness to adapt to the changing environment and the ability to develop a better relationship with the customers, through the business changes. Considering the framework components for the cooperation of entities in tourism, the forms of business connections and modalities in the field of travel agencies and hotel companies are being analysed. This thesis is divided into nine chapters that are presented in a coherent order, from the introduction of the issue to the analysis of the issue itself.