Transfer prices are the prices at which transfers or transactions of products, goods and/or services take place between affiliated companies operating within the same group or centers of responsibility within one business system. The main role of transfer pricing is to meet certain goals of the corporations / companies, which are primarily reduced to: profit maximization, realization of competitive advantages and increased market share. The process of globalization and the abolition of borders of free movement capital have intensified the use of transfer pricing in international business. Trend of greater use of transfer pricing is also observed in national frameworks where it has recognized potential, especially in the area of tax planning. The issue of transfer pricing and the method used in their calculation is very common in all national and international accounting, tax and financial regulations; the issue has been identified as an interesting research problem in compliance with their application. Despite the fact that there are generally accepted guidelines in the form of standards OECD regulating the use of transferable prices, a large number of endogenous and exogenous factors which affect the real world application of certain methods of calculating transfer prices at the national level. Therefore, the aim of this paper is to perform a comparative analysis of preferred transfer pricing methods and determine the level of harmonization of transfer pricing regulation between EU member states. Transfer pricing regulation was analyzed based on several influential factors: application of the OECD guidelines on transfer pricing, preferred and most commonly used methods of transfer pricing, the application of the Advanced Pricing Agreement (APA) system and timing the duration of the concluded transfer price agreement. Most EU member states implement the principle of independence in determining transfer prices, and in regulating transfer prices it is noted that the legal framework largely depends on the provisions of the law and regulations on income tax. The results of the research showed that a high level of harmonization of the transfer prices of the regulatory framework has been achieved between the countries of the European Union and that they differ the most in terms of the duration of the concluded transfer price agreements.