The subjects of this paper is business negotiation in African countries. Business negotiation is a complex and lengthy process in which two negotiating parties are trying to achieve certain business agreement. International business negotiation is more complex process than business negotiation which is lead within national borders because it is influenced by various factors that should be taken into account so that business negotiation can be completed successfully. In the process of negotiating, cultural differences are visible with regard to the aim of negotiating, negotiating attitude, the style of negotiation, verbal and nonverbal communication, time dimension of negotiation, the use of emotion, form of contract and its elements, decision making and taking risks. The paper presents a comparative overview of cultural differences in international business negotiations in the countries of North Africa (Algeria, Egypt and Morocco) and countries of Sub-Saharan Africa (Nigeria, South Africa and Kenya) in communication and cultural differences. Based on the comparison of ways of business negotiation in these African countries it can be concluded that there are differences between the countries of North Africa and the countries of Sub-Saharan Africa, but also among the African countries individually. Business negotiators should be aware of communication and cultural differences; otherwise the business negotiations can be interrupted and can undermine business reputation of negotiator and organization he represents.