The paper analyzes activity and economic and social impact of trade unions and trade union negotiations in the Republic of Croatia. Paper describes historical development of trade unions and their struggle for labor rights and constant efforts to improve working conditions. Also, paper deals with problems of workers and trade unions in a contemporary social and economic environment. The conclusion of this analysis is that the trade unions impact in the Republic of Croatia is gradually weakening, primarily due to the lack of adjustment to modern market conditions and trends. It is therefore necessary to carry out reforms and revitalize the unions in order to achieve important goals, which primarily concern the strengthening of the role, power and influence of trade unions in the Republic of Croatia. The revitalization of the trade union implies a more active involvement in the creation of economic policy, the orientation towards the traditional unintentionalized labor force, ie the different interest groups, the provision of a wider range of services, new ways of organizing, contemporary access to guidance and further education and development of syndication leadership.