The role of the human factor in business, particularly in the hotel industry, is crucial for the success of an enterprise. Employee motivation, as a key aspect of the human factor, stands out as a decisive factor in achieving success. While theoretical approaches consider various motivation strategies, such as financial incentives or non-monetary rewards, empirical research provides concrete insights into the application of these strategies in real business environments. This process requires careful monitoring to avoid negative consequences, such as demotivation leading to turnover. The role of managers is crucial in understanding the individual needs of employees and creating motivational strategies that enhance their productivity and engagement. The psychological impact that motivation has on employees will reflect on the future success of the business. Increasing employee satisfaction through proper balancing of motivational factors results in long-term stability and competitiveness of the company in the market. A study conducted in a hotel in Dubrovnik provides detailed insights into the practical application of theoretical concepts of motivation. Through the analysis of a sample of 82 employees, their satisfaction with working conditions, and the identification of key motivational factors, the research illustrates the importance of adapting motivational strategies to the specific needs and context of the company. This research underscores the importance of investing in human resources as a key factor in competitiveness and the long-term success of the company.